FastQCFastQC Report
Sun 20 Nov 2022
CO_010-2-1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCO_010-2-1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5606
Sequences flagged as poor quality0
Sequence length25-329
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG2284.067070995362112No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGAC2243.995718872636461No Hit
CGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG1763.139493399928648No Hit
GGTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG1753.121655369247235No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCG1662.96111309311452No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGA1562.7827327863003926No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGACC1442.5686764181234394No Hit
TCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG1372.4438102033535496No Hit
CGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG1041.8551551908669281No Hit
TTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG891.587584730645737No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCG881.569746699964324No Hit
CGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT871.5519086692829112No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAA811.4448804851944346No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCG711.2665001783803067No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAA671.1951480556546556No Hit
ATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT671.1951480556546556No Hit
GTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT661.1773100249732429No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAAT651.1594719942918301No Hit
ATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG651.1594719942918301No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGC641.1416339636104174No Hit
GAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG611.088119871566179No Hit
AATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG601.0702818408847663No Hit
TTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT571.016767748840528No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGACC560.9989297181591152No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAAT550.9810916874777025No Hit
GCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG530.945415626114877No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGC520.9275775954334641No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCAT520.9275775954334641No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATT520.9275775954334641No Hit
TGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT520.9275775954334641No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGG500.8919015340706385No Hit
GCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT490.8740635033892259No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCG480.856225472707813No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGAC460.8205494113449875No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATT460.8205494113449875No Hit
CATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT460.8205494113449875No Hit
GAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT440.784873349982162No Hit
AATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT440.784873349982162No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCA440.784873349982162No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTC430.7670353193007492No Hit
CCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT430.7670353193007492No Hit
TCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT400.7135212272565109No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGG400.7135212272565109No Hit
ATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT390.6956831965750981No Hit
CGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT390.6956831965750981No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATG350.6243310738494471No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCA340.6064930431680342No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGA340.6064930431680342No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTC320.5708169818052087No Hit
GGTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT320.5708169818052087No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCG290.5173028897609704No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGG270.4816268283981448No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGA270.4816268283981448No Hit
GCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT260.46378879771673204No Hit
CCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT260.46378879771673204No Hit
TCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT240.4281127363539065No Hit
CGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT230.41027470567249374No Hit
TCCATGGCCGCCTCCCTAATGTTACACACTGACTAG230.41027470567249374No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCC230.41027470567249374No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGG220.392436674991081No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGA220.392436674991081No Hit
TGGCCGCCTCCCTAATGTTACACACTGACTAG210.3745986443096682No Hit
GCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT210.3745986443096682No Hit
GGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT200.35676061362825545No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGC200.35676061362825545No Hit
ATGGCCGCCTCCCTAATGTTACACACTGACTAG180.3210845522654299No Hit
CATGGCCGCCTCCCTAATGTTACACACTGACTAG180.3210845522654299No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATG170.3032465215840171No Hit
CCATGGCCGCCTCCCTAATGTTACACACTGACTAG160.28540849090260434No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCAT150.2675704602211916No Hit
CCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT150.2675704602211916No Hit
CTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT150.2675704602211916No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAG140.2497324295397788No Hit
TCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT120.21405636817695325No Hit
GGCCGCCTCCCTAATGTTACACACTGACTAG110.1962183374955405No Hit
CGGTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG90.16054227613271496No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGC90.16054227613271496No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCC80.14270424545130217No Hit
ACAGCCGACATACATCCCAGACGCTATAAGCTGTACGTGCTTTTACCGTC80.14270424545130217No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGC80.14270424545130217No Hit
CCGCCTCCCTAATGTTACACACTGACTAG70.1248662147698894No Hit
ACCGACTGCCCATAGAGAGGCTGAGACTGCCAAGGCACACAGGGGATAGG70.1248662147698894No Hit
CCTAATGTTACACACTGACTAGAGACTAGTGGCAAT60.10702818408847663No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)